Wound healing potential of collagen bilayer dressing on infected dermal wound- A study on rat animal model

In the current study, a chemically modified collagen bilayer dressing with ciprofloxacin was prepared from type-I collagen. The modified collagen was reconstituted into a membrane and sponge and the drug Ciprofloxacin-HCl was incorporated and fabricated into a bilayer dressing. In vitro release pattern of the dressing was analysed in PBS which recorded release for 6 days. The efficacy of the dressing was checked on infected wound in rat model and compared with that of open infected wound and control group. The healing pattern was analyzed on days 3, 5, 7, 14 and 21 by wound healing rate, biochemical and histological examinations of granulation tissue samples. Integrin expression on the healing wound was analysed using immunohistochemistry and western blotting analysis. In vivo analysis showed significant wound closure, biochemical analysis such as protein, DNA, hydroxyproline, uronic acid, hexoseamine, SOD and catalase from the granulation tissue, showed enhanced healing in the group treated with collagen bilayer dressing with ciprofloxacin. Integrin expression was more in initial stages of healing than the other two groups confirming enhanced healing in treatment group. Histological analysis and wound closure further confirmed proper healing in this group. Our results suggest that sustained bilayer dressing with drug is efficient in controlling and eliminating the bacterial population at the wound site thereby enhancing the healing.